Sample Type Cell Lysate, Tissue Extracts, Urine, Serum, Plasma, Other biological fluids
Species All
Method of Detection Colorimetric, OD 450 nm
Assay Type Quantitative
Application A plate based colorimetric assay to quantify β-hydroxybutyrate in biological samples as low as 10 μM.
Sensitivity 10 μM
Storage Conditions -20°C
Shipping Temperature Gel Pack
Shelf Life One year from the date of delivery
DESCRIPTION
β-hydroxybutyrate (BHB) is a ketone body produced during periods of prolonged fasting, low carbohydrate intake, or intense exercise. BHB serves as an alternative energy source for various tissues, including brain, heart, and skeletal muscle, when glucose availability is limited. BHB also acts as a signaling molecule, affecting gene expression, oxidative stress, inflammation and other cellular processes. AkrivisBio’s β-hydroxybutyrate assay is a simple, sensitive method of determining BHB content in samples as low as 10 μM. In the assay, BHB is oxidized to acetoacetate forming NADH, which then reduces a nearly colorless tetrazolium to a highly colored formazan measured at OD 450 nm. The measurement of β-hydroxybutyrate levels is often used to assess ketosis, a metabolic state characterized by increased production and utilization of ketone bodies.
