Glyoxalase I Activity Assay (C), Catalog: MA-0425

Catalog MA-0425

Size 100 wells

Sample Type Cell & Tissue Lysates

Method of Detection UV Absorbance: 240 nm

Application A simple, sensitive way to measure Glyoxalase I (Glo1) activity in a variety of samples.

Sensitivity < 0.05 mU of GloI per well 

Storage Conditions -20°C

Shipping Temperature Gel Pack

Shelf Life One year from the date of delivery

Glyoxalase I (Glo1) is responsible for cellular detoxification, specifically protecting cells from methylglyoxal (MG) toxicity. MG is a highly reactive carbonyl compound formed primarily as a byproduct of glycolysis. Due to MG’s extreme cytotoxicity, it spontaneously reacts with proteins, lipids, and DNA to form advanced glycation end-products (AGEs). Glo1 catalyzes the first step of the glyoxalase pathway, converting MG and reduced glutathione into S-D-lactoylglutathione, which is later converted to non-toxic D-lactate. Without adequate Glo1 activity, MG accumulation leads to profound damage of cellular infrastructure and induces oxidative stress and apoptosis. In diabetes, chronic hyperglycemia drives overproduction of MG resulting in diabetic complications such as nephropathy, retinopathy and neuropathy. AkrivisBio’s Glyoxalase I Activity Assay is a simple, sensitive way to measure GloI activity in a variety of samples with a sensitivity below 0.05 mU of GloI per well.