Method of Detection Colorimetric, OD 570 nm, Fluorometric, Ex/Em = 535/587 nm
Application A simple, sensitive way to measure Lysophosphatidylcholine levels in biological samples.
Sensitivity < 10 picomoles per well
Storage Conditions -20°C
Shipping Temperature Gel Pack
Shelf Life One year from the date of delivery
DESCRIPTION
Lysophosphatidylcholine (LPC), also called lysolecithin, is a phospholipid consisting of a glycerol backbone, phosphorylcholine attached at carbon 1 and a single fatty acid esterified at position 2 or 3. It is typically formed by the action on phospholipase A2 on phosphatidylcholine. LPC is a bioactive lipid, acting as a metabolic intermediate in membrane metabolism, a signaling molecule and a mediator in various disease states. The most abundant lysophospholipid in serum, its concentration rises significantly with inflammation or injury. LPC can be reacylated to phosphatidylcholine or degraded to lysophosphatidic acid. It is a major component of oxidized LDL and involved in atherosclerosis. As a signaling molecule it can operate via GPCR and Toll-like receptors resulting in production of pro-inflammatory cytokines, endothelial dysfunction and vascular smooth muscle apoptosis. Elevated levels are associated with cardiovascular disease, atherosclerosis, diabetes, and certain types of cancer making it a useful biomarker. AkrivisBio’s Lysophosphatidylcholine Assay is a simple sensitive way to measure LPC levels in biological samples with a sensitivity below 10 picomoles per well.
