100 wells, Colorimetric, OD 570 nm, or Fluorometric, Ex/Em = 535/587 nm
PRODUCT SUMMARY
Catalog MA-0400
Size 100 wells
Sample Type Cell Lysate, Tissue Extracts, Urine, Plasma, Serum, Other biological fluids
Method of DetectionColorimetric, OD 570 nm, or Fluorometric, Ex/Em = 535/587 nm
Application A simple sensitive way to measure Glycerophosphorylcholine activity in a range of samples.
Sensitivity < 100 nM
Storage Conditions -20°C
Shipping Temperature Gel Pack
Shelf Life One year from the date of delivery
DESCRIPTION
Glycerophosphorylcholine (GPC), is a water-soluble choline-containing glycerophosphodiester that functions as a choline reservoir and a phospholipid metabolite in cells. Structurally, it consists of a glycerol backbone linked via phosphate to choline, lacking the fatty acyl chains characteristic of phosphatidylcholine. In mammalian tissues, GPC arises mainly from the catabolism of phosphatidylcholine and lysophosphatidylcholine, and it can be further hydrolyzed by glycerophosphodiester phosphodiesterases to yield free choline and glycerol‑3‑phosphate, linking it directly to both choline and glycerolipid metabolism. GPC is one of the major intracellular storage forms of choline, alongside phosphocholine, buffering choline availability for membrane phospholipid synthesis and acetylcholine production. GPC is recognized as one of the principal organic osmolytes in renal medullary cells, where its intracellular concentration changes in parallel with extracellular tonicity to protect proteins and membranes from hyperosmotic stress. AkrivisBio’s Glycerophosphorylcholine Assay is a simple sensitive way to quantify GPC from a variety of samples., with a sensitivity below 100 nM.
