Protein Disulfide Isomerase Assay, Catalog: MA-0386

Catalog MA-0386

Size 100 wells

Sample Type Cell & Tissue Culture Lysates, Purified Enzyme Preparations

Method of Detection Fluorometric, Ex/Em = 490/580 nm

Assay Type Quantitative

Application A plate based fluorometric assay to measure protein disulfide isomerase activity in samples.

Sensitivity < 0.1 mU

Storage Conditions -20°C

Shipping Temperature Gel Pack

Shelf Life One year from the date of delivery

Protein Disulfide Isomerase (PDI) is a family of proteins, typically found in the lumen of the endoplasmic reticulum, that catalyze the rearrangement of disulfide bonds in proteins, playing a role in protein folding, and management of oxidative stress. PDI activity is essential for cancer cell survival and proliferation, and inhibition of PDI leads to ER stress in cancer cells. AkrivisBio’s Protein Disulfide Isomerase Activity Assay is a simple, sensitive way to measure PDI activity by taking advantage of its reductase activity which causes the cleavage of a synthetic highly quenched substrate, resulting in a dramatic increase in fluorescence in the presence of PDI activity, with a sensitivity below 0.1 mU.