Glycoprotein O-GalNAc (N-Acetylgalactosamine) Labeling and Detection Assay, Catalog: MA-0396

Catalog MA-0396

Size 100 tests

Sample Type Adherent & Suspension Cells

Method of Detection Green Fluorescence (Ex/Em = 492/517), FACS/Microscopy

Application A simple, specific method of attaching GalNAc to proteins within active cells and subsequently labeling them to allow for their detection.

Storage Conditions -20°C

Shipping Temperature Gel Pack

Shelf Life One year from the date of delivery

Glycosylation of proteins is a common cellular mechanism to sort proteins for membrane attachment or secretion or to designate them for other specific purposes. O-linked mucin-type glycans are assembled, starting with the addition of N-acetylgalactosamine to a Serine or Threonine residue of a newly formed protein. This (Tn antigen) structure can be further modified by addition of either galactose, forming GalGalNAc (core1), sialic acid,  forming sialyl-Tn or N-acetylglucosamine, forming GlcNAcGalNAc core3 structures, respectively. Core1 and core3 structures are then extended with the addition of further components, forming long, branched complex O-glycans. AkrivisBio’s Glycoprotein GalNAc Labeling and Detection Assay is a simple specific method of attaching GalNAc to proteins within active cells and subsequently labeling them to allow for their detection.