Cytidine Deaminase Activity Assay, Catalog: MA-0385

Catalog MA-0385

Size 100 wells

Sample Type Tissue Extract, Cell Lysate, Nuclear Extract, Purified Recombinant Protein

Method of Detection Fluorometric, Ex/Em = 410/470 nm

Assay Type Quantitative

Application A simple, sensitive plate-based fluorometric assay to monitor Cytidine Deaminase activity in a variety of biological sample types. 

Sensitivity < 1 µUnit per well

Storage Conditions -20°C

Shipping Temperature Gel Pack

Shelf Life One year from the date of delivery

Cytidine deaminase (CDA) is an essential part of the pyrimidine salvage pathway, deaminating cytidine and deoxycytidine to uridine and deoxyuridine. This allows the recycling of nucleotides. CDA facilitates mitochondria biogenesis and oxphos and in some cancers where CDA activity is suppressed, mitochondrial respiration declines and glycolysis is increased, one of the classic biomarkers of cancer. Nucleoside analog drugs such as gemcitabine and cytaridine are rapidly inactivated by CDA giving rise to chemoresistance. Bloom syndrome is a condition where CDA deficiency or mutation results in replicative stress and genomic instability. CDA dysregulation also can result in immunodeficiencies and other metabolic disturbances. AkrivisBio’s Cytidine Deaminase Activity Assay is a simple, sensitive means of monitoring CDA activity in a variety of biological sample types with a sensitivity below 1 µUnit per well.