Alanyl Aminopeptidase Activity Assay (F), Catalog: MA-0384

Catalog MA-0384

Size 100 wells

Sample Type Tissue Extract, Cell Lysate, Serum, Plasma, Other Biological Fluids

Method of Detection Fluorometric, Ex/Em = 340/440 nm 

Assay Type Quantitative

Application A simple, sensitive plate-based fluorometric assay to monitor Alanyl Aminopeptidase enzyme activity in biological samples.

Sensitivity < 100 µUnits per well

Storage Conditions -20°C

Shipping Temperature Gel Pack

Shelf Life One year from the date of delivery

Alanyl aminopeptidase, also known as aminopeptidase N, APN and CD13 is a Zn²⁺-dependent membrane-bound enzyme, found primarily in the small intestine, kidney, immune cells and vascular endothelium. Its main function is the removal of neutral N-terminal amino acids, from peptides. It is involved in a variety of roles including: a) hydrolyzing peptides that regulate vascular tone and kidney function, b) as a cell surface marker on myelomonocytic cells and expressed on activated T-cells, suggesting a modulatory role in immune response, c) as a cellular receptor for several coronaviruses and d) participation in antioxidant activities and detoxification and inflammation pathways and GSH metabolism. Dysregulation of alanyl aminopeptidase is associated with colorectal cancer, leukemia, lymphoma, hypertension, kidney disorders, neurodegenerative diseases and autoimmunity and inflammatory conditions. AkrivisBio’s Alanyl Aminopeptidase Activity Assay is a simple, sensitive way to monitor enzyme activity in biological samples with a sensitivity below 100 µUnits per well.