α-Glucosidase Activity Assay, Catalog: MA-0376

Catalog MA-0376

Size 100 wells

Sample Type Tissue Lysate, Cell Lysate, Serum, Saliva

Method of Detection Colorimetric, OD 410 nm

Assay Type Quantitative

Application A simple, sensitive plate-based colorimetric assay to measure α-Glucosidase Activity in a variety of sample types.

Sensitivity < below 100 µUnits per well

Storage Conditions -20°C

Shipping Temperature Gel Pack

Shelf Life One year from the date of delivery

α-Glucosidase, primarily located in the brush-border of the small intestine, catalyzes the final step of starch and disaccharide digestion by hydrolyzing terminal, non-reducing α-1,4-glucoosidic linkages to release α-D glucose. This process is vital for the conversion of complex dietary carbohydrates into absorbable monosaccharides, thereby directly influencing blood glucose levels and overall energy homeostasis. A specific form, acid α-glucosidase is located in lysosomes. Deficiency in this enzyme is responsible for Pompe disease. Inhibition of this enzyme using acarbose or miglitol is an important method for managing type-2 diabetes and are being explored for weight management and cardiovascular risk factors. AkrivisBio’s α-Glucosidase Activity Assay is a simple, sensitive means of measuring enzyme activity from a variety of biological sample types with a sensitivity below 100 µUnits per well.