96 wells/40 samples with or 80 samples without background correction; Colorimetric, OD 570 nm
PRODUCT SUMMARY
CatalogRP-0107C
Size96 wells/40 samples with or 80 samples without background correction
Sample Type Cell & Tissue Lysates
Method of DetectionColorimetric, OD 570 nm
Assay Type Quantitative
Application For the detection of Pyruvate Kinase activity from a variety of sample types.
Sensitivity < 50 μU
Storage Conditions -20°C
Shipping Temperature Gel Pack
Shelf Life Six months from the date of delivery
DESCRIPTION
Pyruvate kinase (PK) is a key enzyme in the glycolytic pathway, catalyzing the final step, conversion of phosphoenolpyruvate (PEP) to pyruvate forming ATP. This reaction serves as a key control point, regulating the overall rate of glycolysis and determining the fate of glucose metabolism. PK plays a central role providing energy for cellular processes, as pyruvate is a metabolite common to several pathways. PK also contributes to lactate production under anaerobiosis and influences the balance between oxidative phosphorylation and anaerobic glycolysis, a highly important metabolic choice. AkrivisBio’s Pyruvate Kinase Assay provides a simple, sensitive method for determining PK activity, from a variety of sample types down to below 50 μU.
