Phospholipase A2 Activity Assay, Catalog: MA-0341

Catalog MA-0341

Size 100 wells

Sample Type Tissue Lysates, Cell Lysates

Method of Detection Fluorometric, Ex/Em = 388/513 nm

Assay Type Quantitative

Application A simple, sensitive plate based fluorometric assay to measure Phospholipase A₂ activity in a variety of  biological samples.

Sensitivity < 0.1 mU

Storage Conditions -20°C

Shipping Temperature Gel Pack

Shelf Life One year from the date of delivery

Phospholipases A₂ are a family of enzymes that catalyze the hydrolysis of the sn-2 fatty acid of a glycerophospholipid releasing a lysophospholipid and the free fatty acid. The PLA₂ superfamily can be divided into three major types based upon size and cation dependence. Two subtypes, the low MW secretory  enzymes (sPLA₂) and the high MW cytosolic enzymes (cPLA₂), require Ca²⁺ for activity. sPLA₂ enzymes play an important role in the digestion of dietary phospholipids,  defend against bacterial infections and release free eicosanoids. cPLA₂ enzymes are involved in in inflammation, releasing polyunsaturated fatty acids from membrane lipids allowing production of a host of oxidized eicosanoids. The third type, high MW CA²⁺-independent enzymes (iPLA₂), are intracellular enzymes that appear to be involved in phospholipid remodeling, cell proliferation and fat catabolism. AkrivisBio’s Phospholipase A₂ Activity Assay provides a simple, sensitive way to measure PLA₂ activity in a variety of  biological samples with a sensitivity below 0.1 mU.