6-Phosphogluconate Dehydrogenase Activity Assay, Catalog: MA-0337

Catalog MA-0337

Size 100 wells

Sample Type Purified Enzyme, Tissue & Cell Lysates

Method of Detection Colorimetric, OD 450 nm

Assay Type Quantitative

Application A simple, sensitive plate based colorimetric assay to measure 6-Phosphogluconate Dehydrogenase activity in various biological samples.

Sensitivity < 50 µUnits per well

Storage Conditions -20°C

Shipping Temperature Gel Pack

Shelf Life One year from the date of delivery

6-Phosphogluconate dehydrogenase a step in the hexose monophosphate shunt and pentose phosphate pathways, catalyzes the oxidative decarboxylation of 6-phosphogluconate to ribulose-5-phosphate with reduction of NADP to NADPH. Ribulose-5-phosphate is a precursor of nucleotides and nucleic acids and is reversibly converted to glyceraldehyde-3-phosphate and fructose-6-phosphate to enter glycolysis. It is an important source of NADPH, critical for biosynthetic pathways and prevention of oxidative damage. In humans, its deficiency results in depletion of NADPH in red blood cells. 6-PGD deficiency increases the risk of hemolytic anemia during oxidative stress. Inhibition of the enzyme shows anti-cancer activity, decreasing RNA biosynthesis and increasing ROS in tumor cells. AkrivisBio’s 6-Phosphogluconate DH Activity Assay is a simple, sensitive way to measure 6-PGD activity in various biological samples with a sensitivity below 50 µUnits per well.