Sample Type Purified Protein, Plant, Bacterial Samples
Method of DetectionColorimetric, OD 570 nm
Assay Type Quantitative
Application A plate-based colorimetric assay to measure Phosphoenolpyruvate Carboxylase activity in biological samples.
Sensitivity < 200 µUnits per well
Storage Conditions -20°C
Shipping Temperature Gel Pack
Shelf Life One year from the date of delivery
DESCRIPTION
Phosphoenolpyruvate carboxylase (PEPC, EC 4.1.1.31) is a highly regulated enzyme found in plants, bacteria, and some protists, though notably absent in fungi and animals. It plays a central metabolic role, catalyzing the irreversible carboxylation of phosphoenolpyruvate, forming oxaloacetate, important for CO2 fixation, pH regulation, and supply of the carbon backbone for biosynthetic pathways. In C4 plants, PEPC fixes CO2 into malate or aspartate. It has an anaplerotic role in all plants, replenishing TCA cycle intermediates used in metabolic reactions. AkrivisBio’s Phosphoenolpyruvate Carboxylase Activity Assay is a simple, sensitive way to measure PEPC activity in biological samples with a sensitivity below 200 µUnits per well.
