Nitric Oxide Synthase Activity Assay, Catalog: MA-0327

Catalog MA-0327

Size 100 wells

Sample Type Purified Recombinant Protein, Tissue or Cell Extracts

Method of Detection Colorimetric, OD 540 nm

Assay Type Quantitative

Application A simple, sensitive plate-based colorimetric method of measuring NOS activity in different biological specimens.

Sensitivity < 5 µUnits per well

Storage Conditions -80°C

Shipping Temperature Dry Ice

Shelf Life One year from the date of delivery

Nitric oxide synthase (NOS) is a family of enzymes that produce nitric oxide (NO) from L-arginine, important for several physiological  processes, including neurotransmission, vascular tone, and the immune response. NOS catalyzes an unusual five-electron oxidation of arginine, forming NO and L-citrulline. There are three main NOS isoforms of NOS, neuronal (nNOS), endothelial (eNOS), and inducible (iNOS). nNOS is calcium and calmodulin-dependent and produces NO in the central nervous system. eNOS, also calcium and calmodulin-dependent, produces NO in blood vessels and regulates vascular tone. iNOS is expressed in response to inflammatory stimuli and produces large amounts of NO as a part of the immune response. AkrivisBio’s Nitric Oxide Synthase Activity Assay is a simple, sensitive method of measuring NOS activity in different biological specimens, with a sensitivity below 5 µUnits per well.