Nitric Oxide Assay (F), Catalog: MA-0326

Catalog MA-0326

Size 200 wells

Sample Type Cell & Tissue Lysates, Culture Media, Urine, Plasma, Serum, Other Biological Fluids

Method of Detection Fluorometric, Ex/Em = 360/450 nm 

Assay Type Quantitative

Application A simple, sensitive plate-based fluorometric way to measure nitrite in biological samples, providing an indirect measure of NO in various tissues.

Storage Conditions -20°C

Shipping Temperature Gel Pack

Shelf Life One year from the date of delivery

Nitric oxide (NO), a short-lived gaseous messenger molecule, influences vascular health, mitochondrial function, cellular energy balance and immune activity. At low levels, it enhances glucose and fatty acid oxidation stimulating energy production and facilitating mitochondrial biogenesis. At high concentrations  it inhibits mitochondrial enzymes shifting metabolism toward glycolysis and impairing ATP production. This dual role allows it to function as a metabolic rheostat. NO is rapidly converted to nitrite and nitrate so measurement of total nitrite plys nitrate is a way to measure overall NO production. Using nitrate reductase to convert nitrate to nitrite allows total nitrite to serve as a direct indicator of NO production. AkrivisBio’s Nitric Oxide Assay is a simple, sensitive way to measure nitrite in biological samples, providing an indirect measure of NO in various tissues.