PicoSense™ NADPH Assay, Catalog: MA-0324

Catalog MA-0324

Size 100 wells

Sample Type Tissue Lysate, Cell Lysate, Mitochondria

Method of Detection  Fluorometric, Ex/Em = 535/587 nm

Assay Type Quantitative

Application A rapid and simple plate-based fluorometric assay to detect intracellular NADPH. It does not require purifying NADPH from samples. 

Sensitivity < 40 nM NADPH in a variety of samples.

Storage Conditions -20°C

Shipping Temperature Gel Pack

Shelf Life One year from the date of delivery

NADP (nicotinamide dinucleotide) is a major electron donor for anabolic reactions such as lipid and nucleic acid synthesis. The reduced form, NADPH is a cofactor for many redox enzymes. It is of increasing interest to be able to measure low level of intracellular NADPH. AkrivisBio’s PicoProbe™ NADPH Quantitation Assay kit provides a highly sensitive method to detect intracellular NADPH. NADH is not detected as the NADPH Recycling Enzyme Mix specifically recognizes NADPH in the enzyme recycling reaction. The assay is rapid, simple and does not require purifying NADPH from samples. The kit can measure less than 40 nM NADPH in a variety of samples.