NADH Assay (F), Catalog: MA-0323

Catalog MA-0323

Size 100 wells

Sample Type Tissue Lysate, Cell Extracts, Enzymatic reactions

Method of Detection Fluorometric, Ex/Em = 535/587 nm

Assay Type Quantitative

Application A simple, sensitive plate-based fluorometric assay to measure NADH and/or NAD in a variety of sample types.

Sensitivity In the very low nanomolar range

Storage Conditions -20°C

Shipping Temperature Gel Pack

Shelf Life One year from the date of delivery

The NAD/NADH pair are the primary electron acceptor and donor, respectively, in all living organisms. Most energy-related catabolic reactions result in the formation of NADH from NAD. The electrons carried by NADH mainly end up at the electron transport chain and result in the formation of ATP the primary energy molecule. THE NA/NADH ratio is tightly regulated, modulating metabolic fluxes and preventing excessive oxidative or reductive stress in cells. Other than a source of energy, NADH is also involved in DNA repair, signaling pathways and a variety of metabolic functions. Alterations in NADH levels or its metabolism are associated with age-related disorders, neurodegeneration, metabolic diseases, and cancer. AkrivisBio’s PicoSense NADH Assay is a simple, sensitive means of measuring NADH and/or NAD in a variety of sample types with a sensitivity in the very low nanomolar range.