Myeloperoxidase Peroxidation Activity Assay, Catalog: MA-0322

Catalog MA-0322

Size 100 wells

Sample Type Cell Lysates, Tissue Extracts

Method of Detection Fluorometric, Ex/Em = 535/587 nm

Assay Type Quantitative

Application A plate based simple, and sensitive fluorometric assay to measure the peroxidatic activity, the transfer of electrons to hydrogen peroxide from a substrate.

Sensitivity < 1 µUnit per well

Storage Conditions -20°C

Shipping Temperature Gel Pack

Shelf Life One year from the date of delivery

Myeloperoxidase (MPO), produced by neutrophils and monocytes, is involved in the innate immune response and pathogenesis of several chronic diseases. MPO is released from activated neutrophils during a "respiratory burst" at sites of infection or inflammation. It catalyzes the conversion of H2O2 and chloride ion into hypochlorite, a highly reactive oxidant capable of killing pathogens, a function essential for effective innate immunity. MPO can also modify low-density lipoprotein (promoting uptake by macrophages and contributing to foam cell formation) and high-density lipoprotein (impairing protective functions and promoting vascular inflammation). It also catalyzes the formation of tyrosyl radicals and protein nitrosylation, altering protein structure and function and affecting cellular signaling, protein stability and immune recognition. MPO activity is positively correlated with obesity, insulin resistance, and metabolic syndrome and is implicated in the pathogenesis of rheumatoid arthritis, neurodegenerative disease, liver disease, and certain cancers, making it a target for drug development. AkrivisBIo’s Myeloperoxidase Activity Assay measures the peroxidatic activity, the transfer of electrons to hydrogen peroxide from a substrate with a sensitivity below 1 µUnit per well.