β-glucuronidase Activity Assay, Catalog: MA-0290

Catalog MA-0290

Size 100 wells

Sample Type Cell Lysates, Tissue Extracts, Purified Enzyme

Method of Detection Fluorometric, Ex/Em = 320/445 nm

Assay Type  Quantitative

Application A plate-based simple, sensitive fluorometric assay to measure β-glucuronidase activity in a variety of sample types.

Sensitivity < 1 µUnit

Storage Conditions -20°C

Shipping Temperature Gel Pack

Shelf Life One year from the date of delivery

β-Glucuronidase is a lysosomal enzyme which hydrolyses β-D-glucuronic acid residues from a range of drugs, toxins and other xenobiotics. Phase II detoxification includes the conjugation of glucuronic acid to a variety of molecules, making them more water soluble and easily excreted. β-glucuronidase can reverse this with detrimental results. Bacterial β-glucuronidase in the gut affects enterohepatic circulation of many compounds. It also affects the balance of active vs. inactive hormones such as estrogen with implications for hormone-related conditions such as some cancers. Due to its effect on detoxification, hormone regulation and drug metabolism it is being explored as a target for drug development. AkrivisBio’s β-Glucuronidase Activity Assay is a simple, sensitive method of measuring β-glucuronidase activity in a variety of sample types with a sensitivity below 1 µUnit.