Aconitase Activity Assay, Catalog: MA-0235

Catalog MA-0235

Size 100 wells

Sample Type Cell Lysate or Tissue Lysate

Method of Detection Colorimetric 450 nm 

Assay Type Quantitative

Application A plate based simple, sensitive colorimetric assay to measure Aconitase activity in various biological samples.

Sensitivity < ~100 µU per sample

Storage Conditions 4°C

Shipping Temperature Gel Pack

Shelf Life One year from the date of delivery

Aconitase catalyzes the 2^nd step of the TCA cycle, the reversible isomerization of citrate to isocitrate. Aconitase exists in two forms, a mitochondrial and a cytosolic form also called iron regulatory protein 1. Mitochondrial aconitase plays a direct role in the TCA cycle, while cytosolic aconitase functions in iron homeostasis. The enzyme's activity is dependent on a 4Fe-4S cluster, sensitive to oxidative damage. When iron levels are sufficient, IRP1 acts as aconitase in the cytoplasm. Under low iron conditions or oxidative stress, IRP1 loses its 4Fe-4S cluster and becomes an RNA-binding protein that regulates the stability and translation of mRNAs involved in iron metabolism, like transferrin receptor and ferritin. The dual functionality of aconitase as a metabolic enzyme and an iron-regulatory protein links cellular responses to oxidative stress and iron availability. Several disease states  show decreased aconitase activity, mainly due to oxidative destruction of the 4Fe-4S cluster, including Parkinson’s and Alzheimer’s, and cardiovascular and iron metabolism disorders. AkrivisBio’s Aconitase Activity Assay is a simple, sensitive means of quantifying Aconitase activity with a sensitivity below ~ 100 µU per sample.