Aldolase Assay, Catalog: MA-0176

Catalog MA-0176

Size 100 wells

Sample Type Variety of samples including Serum, Plasma, Cell & Tissue Extracts 

Method of Detection Colorimetric, OD 450 nm 

Assay Type Quantitative

Application A plate based simple, sensitive means of measuring aldolase activity in a variety of biological sample types.

Sensitivity Low microunit range (pmole/minute)

Storage Conditions -20°C

Shipping Temperature Gel Pack

Shelf Life One year from the date of delivery

Aldolase catalyzes the reversible transformation of fructose-1,6-biphosphate to glyceraldehyde-3-phosphate and dihydroxyacetone phosphate, a key step in glycolysis and gluconeogenesis. Aldolase acts as a metabolic switch during glycolysis and gluconeogenesis regulating glucose homeostasis. Aldolase has 3 forms, A, B and C localized in different tissues, A primarily in muscle, B in liver and C in brain, each with distinct physiological roles. Dysregulation of aldolase is seen in cardiovascular and neurodegenerative disease as well as in inherited metabolic disorders (fructose intolerance) and cancer where upregulation supports heightened glycolytic activity (Warburg effect). AkrivisBio’s Aldolase Activity Assay is a simple, sensitive method of measuring aldolase activity in a variety of biological sample types with a sensitivity limit in the low microunit range (pmole/minute).