Purine Nucleoside Phosphorylase Activity Assay, Catalog: MA-0225


100 wells, Fluorometric, Ex/Em 535/587 nm


Catalog MA-0225

Size 100 wells

Sample Type Purified Recombinant Protein, Cell Lysate, Tissue Lysate

Method of Detection Fluorometric, Ex/Em 535/587 nm

Assay Type Quantitative

Application A simple, sensitive means of measuring PNP activity in biological samples.

Sensitivity < 5 nU

Storage Conditions -20°C

Shipping Temperature Gel Pack

Shelf Life One year from the date of delivery


Purine Nucleoside Phosphorylase is involved in the purine salvage pathway, recycling purines for nucleotide synthesis. It catalyzes the phosphorolysis of inosine and guanosine, breaking them down into their respective bases, hypoxanthine and guanine and ribose-1-phosphate. PNP deficiency leads to a rare immunodeficiency disorder with a severe effect upon T-cells while often not affecting B-cells. In the disease, dGTP accumulates in the T-cells, inhibiting ribonucleotide reductase and DNA synthesis, leading to dysfunction and apoptosis. Altered purine metabolism has been observed in some cancers and PNP is a potential target as a therapeutic strategy. AkrivisBio’s Purine Nucleoside Phosphorylase Assay is a simple sensitive means of measuring PNP activity in biological samples with a sensitivity below 5 nU.