Method of Detection Fluorometric, Ex/Em 535/587 nm
Assay Type Quantitative
Application A simple, sensitive means of measuring G6PDH activity in a variety of biological samples.
Sensitivity < 1 µU
Storage Conditions -20°C
Shipping Temperature Gel Pack
Shelf Life One year from the date of delivery
DESCRIPTION
Glucose-6-Phosphate Dehydrogenase catalyzes the first step of the pentose phosphate pathway, converting glucose-6-phosphate to 6-phosphoglucono-δ-lactone while converting NADP to NADPH, needed for a variety of cellular functions. NADPH, important for cholesterol and fatty acid synthesis, is much more important as the source of reducing equivalents to keep glutathione in the reduced form, GSH. G6PDH deficiency is probably the most common enzyme deficiency world-wide with an estimated 400 million people affected, which can lead to several medical conditions such as hemolytic anemia and hyperbilirubinemia which can lead to kernicterus in newborns. There is a genetic advantage to having G6PDH deficiency, making it very difficult for the malaria parasite to survive within the red blood cell. The reduced NADPH levels associated with G6PDH deficiency also leads to other metabolic disorders due to impaired cellular processes dependent upon reductive biosynthesis and detoxification. There is a link to increased susceptibility to diabetes and cardiovascular diseases where oxidative stress plays an important role. AkrivisBio’s G6PDH Activity Assay is a simple, sensitive means of measuring G6PDH activity in a variety of biological samples with a sensitivity below 1 µU.
