CYP3A4 Activity Assay, Catalog: MA-0213


100 wells, Fluorometric, Ex/Em = 385/500 nm


Catalog MA-0213

Size 100 wells

Sample Type Tissue lysate, Cell Lysate, Microsomes

Method of Detection Fluorometric, Ex/Em = 385/500 nm

Assay Type Quantitative

Application A simple sensitive plate based assay to measure native or recombinant CYP3A4 activity in biological samples.

Storage Conditions -20°C

Shipping Temperature Gel Pack

Shelf Life One year from the date of delivery


Cytochrome P450 3A4 is the predominant form of P450, with approximately 30-50% of the total P450 activity in the liver. It is responsible for the phase 1 biotransformation of an extremely wide range of xenobiotics as well as many endogenous compounds. Inhibition of this isoform is responsible for a number of adverse drug reactions and drug/drug interactions. AkrivisBio’s CPY3A4 Assay is a simple, sensitive assay for enzyme activity using a sensitive substrate which is converted to 7-Hydroxy-4-trifluoromethylcoumarin with a dramatic increase in fluorescence at 385/500 nm. Specificity is provided by the addition of ketoconazole to the kit which is an index inhibitor  of CYP3A4.