Phosphoglycerate Mutase Activity Assay, Catalog: MA-0209

Catalog MA-0209

Size 100 wells

Sample Type Cell Lysate, Tissue Extracts

Method of Detection Colorimetric, OD 570 nm

Assay Type Quantitative

Application A plate based simple, and sensitive means of assessing Phosphoglycerate Mutase  enzyme activity in a variety of sample types. 

Sensitivity In the low microunit range.  

Storage Conditions -20°C

Shipping Temperature Gel Pack

Shelf Life One year from the date of delivery

Phosphoglycerate mutase (PGAM), a member of the glycolytic pathway, catalyzes the reversible conversion of 3-phosphoglycerate to 2-phosphoglycerate, which occurs in both glycolysis and gluconeogenesis. During glycolysis, 3-PG is produced from dephosphorylation of 1,3- diphosphoglycerate. Phosphoglycerate mutase then converts 3-PG to 2-PG, allowing glycolysis to proceed, maintaining the flow of metabolites, leading to pyruvate which enters the TCA cycle powering oxidative phosphorylation. In gluconeogenesis, 2-PG is converted to 3-PG, essential  for replenishment of glucose under conditions of low glucose, ensuring supply for tissues with high energy demand such as brain and red blood cells. Dysregulation of PGAM is associated with several medical conditions. It may be upregulated in cancer cells which depend on a high glycolytic flux. AkrivisBio’s Phosphoglycerate Mutase Activity Assay is a simple, sensitive means of assessing enzyme activity with a sensitivity in the low microunit range.