Method of Detection Colorimetric, OD 570 nm / Fluorometric, Ex/Em = 535/587 nm
Assay Type Quantitative
Application A plate based simple and direct means of assessing adipogenesis that has occurred under a wide range of conditions.
Sensitivity <0.5 nmol/well
Storage Conditions -20°C
Shipping Temperature Gel Pack
Shelf Life One year from the date of delivery
DESCRIPTION
Adipogenesis is the process where undifferentiated cells become adipocytes, responsible for energy storage in the form of lipids. The 3T3-L1 cell line is a widely used model for studying adipogenesis due to its ability to undergo differentiation in response to specific stimuli. Adipogenesis can be divided into distinct stages: Commitment, Mitotic Clonal Expansion and terminal Differentiation. Commitment involves induction of transcription factors i.e., CCAAT/EBPβ and C/EBPδ which initiate the expression of PPARγ and C/EBPα, the master regulators of adipocyte differentiation. MCE involves cell cycle progression and division, leading to expansion of preadipocyte populations. Terminal differentiation involves maturation of preadipocytes into functional adipocytes, marked by accumulation of lipid droplets. Adipogenesis in 3T3-L1 cells serves as a useful model for studying adipocyte differentiation. AkrivisBio’s Adipogenesis Assay is a simple, direct means of assessing adipogenesis which has occurred under a wide range of conditions.
