Heme Assay, Catalog: MA-0190

Catalog MA-0190

Size 100 wells

Sample Type Cell culture supernatant, Tissue Extracts, Urine, Serum, Plasma

Method of Detection Colorimetric, OD 570 nm 

Assay Type Quantitative

Application A plate based simple, sensitive colorimetric assay to measure heme content below 5 pg in samples. 

Sensitivity <5 pg in samples

Storage Conditions 4°C

Shipping Temperature Gel Pack

Shelf Life One year from the date of delivery

Heme (iron coordinated to OH-) or hemin (iron coordinated to Cl-) is an iron containing tetrapyrrole produced by the porphyrin synthetic pathway, beginning with ALA synthase. It is also generated by the catabolic breakdown of heme proteins such as myoglobin and hemoglobin. It can be detected in body fluids under several pathological conditions. Free heme exists in cells at very minute concentrations below 100 nM, since it exhibits significant toxicity due to its ability to initiate lipid peroxidation and the formation of ROS species. Heme has valid regulatory purposes, such as inhibition of ALA-synthase in cells other than erythrocytes, repression of ALA-synthase expression at the genetic level and induction of heme oxygenase. Heme stimulates growth of oral bacteria in gingivitis and is an indicator of pathological conditions when found in the urine or feces. AkrivisBio’s Heme Assay is a simple, extremely sensitive way to measure heme content below 5 pg in the colorimetric assay.