Nuclear/Cytosol Fractionation Kit, Catalog: PI-0107
Size 25 or 100 Preparations
Sample Type Cells and Tissues
Assay Type Qualitative
Application To provide an efficient system which enables the separation of nuclear material from the cytoplasmic fraction of mammalian cells with little to no cross-contamination.
Storage Conditions 4°C
Shipping Temperature Gel Pack
Shelf Life One year from the date of delivery
Isolation of nuclear material, free of cytoplasmic contaminants and/or cytoplasmic proteins, free of nuclear components is needed for a range of analytical purposes. AkrivisBio’s Nuclear/Cytosol Fractionation Kit provides an efficient system which enables the separation of nuclear material from the cytoplasmic fraction of mammalian cells with little to no cross-contamination. Our optimized reagents and protocol require only a tabletop centrifuge, sample tubes and pipettors. A complete isolation procedure can be completed in as little as 2 hours. The extracted nuclear and cytoplasmic protein fractions are functional and compatible with a variety of downstream applications such as measurement of transcriptional activity, RNA splicing, gel shift assay, reporter assay, enzyme activity assay, or blotting.