Sample Type Tissue Extracts, Cell Lysate, Purified Mitochondria
Method of Detection Colorimetric, OD 450 nm
Assay Type Quantitative
ApplicationA plate based simple, sensitive colorimetric assay to measure Pyruvate Dehydrogenase (PDH) activity with a sensitivity limit below 50 µU.
Sensitivity <50 µU
Storage Conditions -20°C
Shipping Temperature Gel Pack
Shelf Life One year from the date of delivery
DESCRIPTION
Pyruvate Dehydrogenase (PDH), a multienzyme complex, catalyzes the oxidative decarboxylation of pyruvate, producing acetyl-CoA, NADH and CO2. PDH connects the TCA cycle and oxidative phosphorylation to glycolysis, gluconeogenesis, and lipid and amino acid metabolic pathways. The activity of PDH is regulated through reversible phosphorylation of the E1-alpha subunit at specific serine sites. Phosphorylation, catalyzed by PDH kinases 1-4, decreases PDH activity. Dephosphorylation, catalyzed by PDP1 and 2, restores PDH activity. Multiple regulatory signals control PDH activity. Dysregulation is associated with metabolic disorders like diabetes, obesity and cancer. Genetic defects in the PDH complex lead to lactic acidosis and Leigh’s disease among others. AkrivisBio’s Pyruvate Dehydrogenase Activity Assay is a simple, sensitive way to measure PDH activity with a sensitivity limit below 50 µU.
