Sample Type Tissue Extracts, Cell Lysate, Serum, Plasma, Urine, Other biological fluids
Method of Detection Colorimetric, OD 570 nm or Fluorometric, Ex/Em = 535/587 nm
Assay Type Quantitative
Application A plate based colorimetric/fluorometric assay to measure free L-Carnitine in biological samples.
Sensitivity 0.2 nmoles/well
Storage Conditions -20°C
Shipping Temperature Gel Pack
Shelf Life One year from the date of delivery
DESCRIPTION
Carnitine is a critical transport molecule which shuttles fatty acids into the mitochondria where they are utilized to make acetyl CoA, a key part of the TCA cycle. In the reverse direction carnitine shuttles toxic by-products of fatty acid metabolism out of the mitochondria, minimizing oxidative stress and oxidative damage that would otherwise occur. L-Carnitine is synthesized from methionine and lysine. Beyond its highly important shuttle function, it also enhances insulin sensitivity and cellular glucose uptake. AkrivisBio’s L-Carnitine Assay is a simple, sensitive means of measuring free L-Carnitine in biological samples.
