Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Activity Assay, Catalog: MA-0153

Catalog MA-0153

Size 100 wells

Sample Type Cell Lysate, Tissue Extracts

Method of Detection Colorimetric, OD 450 nm 

Assay Type Quantitative

Application A plate based colorimetric assay to measure GAPDH activity in a variety of samples with a sensitivity into the low µU/ml. 

Sensitivity Low µU/ml or 0.4 nmoles/well

Storage Conditions -20°C

Shipping Temperature Gel Pack

Shelf Life One year from the date of delivery

GAPDH catalyzes the conversion of glyceraldehyde-3-phosphate to 1, 3-bisphosphoglycerate (1,3-BPG), creating a high-energy phosphate bond while simultaneously facilitating the formation of NADH. This is a key step in glycolysis. 1,3-BPG goes on to result in ATP production while the NADH powers the electron transport chain, synthesizing more ATP. GAPDH is an essential constitutive enzyme whose activity is regulated by several factors, including cellular energy demand, redox state and post-translational modifications. Dysregulation of GAPDH is associated with cancer and neurodegenerative disorders. AkrivisBio’s GAPDH Assay is a simple, sensitive means of measuring GAPDH activity in a variety of samples with a sensitivity into the low µU/ml.