Method of Detection Colorimetric, OD 570 nm or Fluorometric, Ex/Em = 535/580 nm
Assay Type Quantitative
Application A plate based colorimetric/fluorometric assay to measure ethanol concentration in a variety of samples.
Sensitivity 0.05 nmol
Storage Conditions -20°C
Shipping Temperature Gel Pack
Shelf Life One year from the date of delivery
DESCRIPTION
Ethanol plays a role in various metabolic pathways within the human body. When consumed, it is metabolized primarily in the liver via oxidative metabolism, involving alcohol dehydrogenase and acetaldehyde dehydrogenase, converting ethanol through toxic acetaldehyde, to acetate and eventually CO2 and water. Ethanol metabolism affects energy balance and nutrient utilization. The metabolism of ethanol takes priority over other macronutrients, leading to storage of excess carbohydrates and fats. Chronic alcohol consumption disrupts metabolic pathways, impacting liver function, lipid metabolism, and glucose regulation. AkrivisBio’s Ethanol Assay provides a simple, sensitive method for precise measurement of ethanol in a variety of samples. In the assay, alcohol oxidase oxidizes ethanol forming H2O2 which is used to form resorufin with intense color and fluorescence with sensitivity to 0.05 nmol.
