6-CFDA, Catalog: 2758

Alternate Name 6-Carboxyfluorescein diacetate

Appearance Off-white solid

CAS # 3348-03-6

Molecular Formula C₂₅H₁₆O₉

Molecular Weight 460.39

Purity ≥ 95% by HPLC

Solubility DMF or DMSO

SMILES CC(=O)OC1=CC2=C(C=C1)C3(C4=C(O2)C=C(C=C4)OC(=O)C)C5=C(C=CC(=C5)C(=O)O)C(=O)O3

InChi InChI=1S/C25H16O9/c1-12(26)31-15-4-7-18-21(10-15)33-22-11-16(32-13(2)27)5-8-19(22)25(18)20-9-14(23(28)29)3-6-17(20)24(30)34-25/h3-11H,1-2H3,(H,28,29)

InChi Key QMOGCCYGOPYYNT-UHFFFAOYSA-N

PubChem CID 151095

MDL Number MFCD00036871

Storage Conditions -20°C

Shipping Conditions Gel Pack

Usage For Research Use Only! Not For Use in Humans.

Handling Do not take it internally. Wear a glove and mask when handling the product. Protect from air, light and moisture.

6-CFDA has been used to differentiate viable cells from apoptotic cells. • Live cells that are not apoptotic accumulate 6-carboxyfluorescein but do not accumulate annexin. CFDA enters cells and is hydrolyzed more slowly than FDA. CFDA and FDA hydrolysis obey Michaelis-Menten kinetics with Km values of around 19 and 2 µM, respectively.