Beta-galactosidase Staining Kit, Catalog: COS-0104

Catalog COS-0104

Size: Includes reagents to stain 20 x 12-well plates or the equivalent volume of 6 or 24-well plates.

Sample Type Adherent Cells, Suspension Cells

Species All

Method of Detection Light Microscopy

Assay Type Qualitative

Application For visualizing β-Galactosidase activity in cells under a light microscope.

Range The response is highly linear between 10000 – 100000 cells per well

Storage Conditions -20°C

Shipping Temperature Gel Pack

Shelf Life One year from the date of delivery

The E. coli LacZ gene is a commonly used reporter gene for testing the expression efficiency of vector mediated gene transfer. It is also used frequently to study gene promoter regulation. LacZ encodes β-galactosidase, an almost perfectly ideal choice for expression due to being very stable, resistant to proteolytic degradation, able to utilize multiple substrates and easily assayed in situ. AkrivisBio’s β-Galactosidase Staining Kit is a simple, sensitive assay, which utilizes X-Gal a colorless substrate, resulting in an intense blue insoluble indigo product easily visualized under microscopy.