Sample Type Cell Lysate, Tissue Extracts, Urine, Serum, Other biological fluids
Species All
Method of Detection Colorimetric, OD 412 nm
Assay Type Quantitative
Application A plate based colorimetric assay to measure the enzymatic activity of MPO in a wide range of sample types.
Sensitivity <0.05 mU/well
Storage Conditions -20°C
Shipping Temperature Gel Pack
Shelf Life One year from the date of delivery
DESCRIPTION
Myeloperoxidase (MPO) is an oxidative enzyme abundantly expressed in neutrophils and is involved in the immune response and inflammation. Additionally, MPO can also oxidize lipids, proteins and DNA, leading to oxidative damage in chronic inflammatory conditions such as atherosclerosis, COPD and neurodegenerative disease. AkrivisBio’s MPO Assay is a simple sensitive means of measuring MPO enzyme activity below 0.05 mU per well, which can be indicative of neutrophil activation and oxidative stress.In the assay, Myeloperoxidase produces HOCl (hypochlorous acid) from hydrogen peroxide and chloride. HOCl reacts with taurine forming taurine chloramine, which then reacts with TNB, the reduced form of DTNB, Ellman’s Reagent, bleaching out the color causing a decrease of absorbance measured at OD 412 nm. The absorbance is inversely proportional to the amount of MPO enzyme.
