Sample Type Cell Lysate, Tissue Extracts, Urine, Serum, Plasma, Other biological fluids
Species All
Method of Detection Colorimetric, OD 570 nm or Fluorometric, Ex/Em = 535/587 nm
Assay Type Quantitative
Application A plate based colorimetric/fluorometric assay to quantify citrate levels in biological samples.
Sensitivity <0.1 nmoles
Storage Conditions -20°C
Shipping Temperature Gel Pack
Shelf Life One year from the date of delivery
DESCRIPTION
Citrate is a metabolic intermediate in the tricarboxylic acid cycle, a key metabolic pathway, responsible for generating energy in the form of ATP. It is also an allosteric modulator of fatty acid synthesis and glycolysis. Decreased citrate levels are associated with kidney stones, metabolic syndrome and prostate cancer. AkrivisBio’s Citrate Assay provides a simple, sensitive means of measuring citrate in a variety of samples with a sensitivity below 0.1 nanomoles. In the assay, citrate is converted to oxaloacetate and then to pyruvate, which is oxidized by pyruvate oxidase forming hydrogen peroxide. Hydrogen peroxide is then utilized by the peroxidase to oxidize ADHP forming an intense color or fluorescence measured at OD = 570 nm or at Ex/Em = 535/587 nm respectively.
