Sample Type Serum, Plasma, Cell Lysate, Tissue Lysate, Urine, Cell Culture Media
Species All
Method of Detection Colorimetric, OD 570 nm
Assay Type Quantitative
Application A plate based colorimetric assay to quantify ammonia below 1 nmol per well in various samples.
Storage Conditions -20°C
Shipping Temperature Gel Pack
Shelf Life One year from the date of delivery
DESCRIPTION
Ammonia is the primary central source of nitrogen for living systems. Ammonia is both a metabolic product and a necessary enzyme substrate for a wide range of reactions. It is created through nitrogen fixation or amino acid deamidation. It’s one of the primary contributors to acid/base balance. AkrivisBio’s Ammonia Assay provides a simple, sensitive procedure for the accurate determination of ammonia below 1 nmol per well. In the assay, Glutamate dehydrogenase converts NADH in the presence of ammonia and α-ketoglutarate to NAD, which converts lactate to pyruvate. The pyruvate is oxidized forming hydrogen peroxide which is used by peroxidase to convert ADHP to resorufin with intense color measured at OD 570 nm.
