Sample Type Serum, Urine, other biological fluids, Cerebral Spinal Fluid
Species All
Method of Detection Colorimetric, OD 570 nm or Fluorometric, Ex/Em = 535/587 nm
Assay Type Quantitative
Application A plate based colorimetric/fluorometric assay to measure glucose concentration in a wide range of biological samples.
Sensitivity 1 µM
Storage Conditions -20°C
Shipping Temperature Gel Pack
Shelf Life One year from the date of delivery
DESCRIPTION
Glucose is the primary source of energy in all organisms. In plants, glucose is stored as starch and as glycogen in animals. Blood glucose concentration is highly regulated and a variety of metabolic disorders is reflected in a significant deviation in glucose concentration. AkrivisBio’s Glucose Assay is a simple, sensitive way to measure Glucose in a wide range of biological samples. In this assay, Glucose is oxidized by glucose oxidase forming gluconolactone and hydrogen peroxide. Hydrogen peroxide is utilized by the peroxidase to oxidize ADHP thereby resulting in the formation of intense color or fluorescence measured at OD 570 nm or at Ex/Em = 535/587 nm respectively.
