LDH Based Cell Cytotoxicity Assay, Catalog: CPT-0101
Size Includes reagents for 500 wells
Sample Type Cells
Species Non specific
Method of Detection Colorimetric, OD 450 nm
Assay Type Quantitative
Application For the detection of LDH released from damaged cells.
Range 20000-100000 cells/100 µl in wells of a 96-well plate
Storage Conditions 4°C
Shipping Temperature Gel Pack
Shelf Life One year from the date of delivery
Cell death or cytotoxicity is classically evaluated by assessing the degree of plasma membrane damage. Lactate dehydrogenase (LDH) is an intracellular enzyme that is released into the extracellular medium upon cellular damage or lysis, making it a useful indicator of cytotoxic effects. AkrivisBio’s LDH Cytotoxicity Assay Kit is used to assess cell membrane integrity and measure cell death or cytotoxicity. The assay utilizes WST, a tetrazolium detection reagent to give a fast, highly sensitive measure of LDH release from damaged cells. The intensity of color correlates directly with the number of damaged cells.