NAD/NADH Assay, Catalog: MA-0114

Catalog MA-0114

Size 100 wells

Sample Type  Tissue Extracts, Cell Lysate, Serum, Urine, other biological fluids

Species All

Method of Detection Colorimetric, OD 450 nm

Assay Type Quantitative

Application A plate based colorimetric assay to measure NAD+ and NADH, and their ratio in various samples.

Sensitivity 400 nM - 2000 nM

Storage Conditions -20°C

Shipping Temperature Gel Pack

Shelf Life One year from the date of delivery

The NAD/NADH redox couple is essential for maintaining cellular redox homeostasis. NADH is the primary vehicle whereby reducing equivalents are converted to ATP in the cell. With thousands of oxidoreductases in the cell, each with its own set of optimal conditions, the assay of nicotinamide nucleotides becomes a useful very broad index of the overall health of the cell. AkrivisBio’s NAD/NADH Assay provides a simple, sensitive means of detecting NAD and NADH. The ability to measure these concentrations allows the determination of the NAD/ NADH ratio, which gives important insight into the overall redox state of the cell. The assay is specific for the NAD/NADH couple and not NADP/ NADPH, which are used primarily for unrelated anabolic reactions.