ATP Assay, Catalog: MA-0113

Catalog MA-0113

Size 100 wells

Sample Type  Tissue Extracts, Cell Lysate, Serum, Plasma, Urine, other biological fluids

Species All

Method of Detection Colorimetric, OD 570 nm or Fluorometric, Ex/Em = 535/587 nm

Assay Type Quantitative

Application A plate based colorimetric/fluorometric assay to quantify the amount of ATP present in various samples. The assay can detect down to 50 pmol (1 µM) of ATP.

Sensitivity <1 µM

Storage Conditions -20°C

Shipping Temperature Gel Pack

Shelf Life One year from the date of delivery

ATP is the primary energy currency of cells. Virtually all energy dependent processes including metabolism, muscle contraction, signal transduction etc. utilize the chemical energy stored in the phosphate bond of ATP. AkrivisBio’s ATP assay kit is simple, robust assay to quantify the amount of ATP present in various samples. In this assay, ATP from samples phosphorylate glycerol. Glycerol phosphate is then oxidized with the formation of hydrogen peroxide, which is then utilized by the peroxidase to oxidize ADHP resulting in the formation of intense color/fluorescence measured at OD 570 nm or at Ex/Em = 535/587 nm.